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nonimmune rabbit serum igg  (Alpha Diagnostics)

 
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    Structured Review

    Alpha Diagnostics nonimmune rabbit serum igg
    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
    Nonimmune Rabbit Serum Igg, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonimmune+rabbit+serum+igg/non+immune+rabbit+or+mouse+igg/pmc10560947-117-12-16
    Average 90 stars, based on 1 article reviews
    nonimmune rabbit serum igg - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy"

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

    Journal: American Journal of Cancer Research

    doi:

    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
    Figure Legend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Techniques Used: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy
    Article Snippet: Chromatin immunoprecipitation sequencing (ChIP-Seq) and real-time qPCR ChIP was performed following the protocol from the Farnham laboratory (http://genomics.ucdavis.edu/farnham). .. Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International). .. Chromatin immunoprecipitation sequencing (ChIP-Seq) and real-time qPCR ChIP was performed following the protocol from the Farnham laboratory (http://genomics.ucdavis.edu/farnham).



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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit <t>IgG</t> and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.
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    Image Search Results


    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Journal: American Journal of Cancer Research

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

    doi:

    Figure Lengend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Article Snippet: Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International).

    Techniques: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR